Algae Investigation In Drinking Water And Source Water And The Methodological Research

In this paper, the existence of algae in the source water and the tap water of Baoding were examined. At the same time, the methods of freshwater algae's cultivation, separation and purification were studied. And the algae cell broken methods were also studied. The results were as follow:1. The kinds of the algae and growth state of the water sumple in 20 kinds of culture media were investigated. With category of algae, the growth circumstance and grow the period for index sign, finally, five better kinds of culture media were found, they were Gargorov-Fitzgerald-Skog culture medium, Microcystis-Anabaena culture medium, Hydrophily 104 nitrogenous culture medium, basic culture medium + glucose and improved Hydrophily 104 nitrogenous culture medium + glucose.2. In this paper the source water and the waterworks water and the tap water of Baoding were examined from March of 2004 to March of 2005. Investigation contained: firstly, the species and quantity of algae in the five water samples were investigated; secondly, the level of eutrophication was evaluated through the content of algae's chlorophyll-a. The result showed the species number and quantity of algae in the source water and the tap water descended dramatically. This indicated that the quality of source water and the tap water in Baoding was becoming better and better.3. Enriched culture, sepration and purification and identification of algae in the source water and tap water in Baoding city. Using culture medium selection, diluted separation, plate lineration, centrifugal separation, capillary separation, droplet separation, pH separation, temperature separation, inhibitor separation and so on, ten species were obtained. There were Cyanophyta Nostoc punctiforme, Phormidium tenue, an undetermined alga; Chrysophyta Chromulina ovalis; Chlorophyta Cholorella ellipsoidea, Scenedesmus bijuga, S. quadricauda, Elakatothrix sp., Selenastrum minutum and Bacillariophyta Navicula sp. In them Nostoc punctiforme, Phormidium tenue, and an undetermined alga, Selenastrum minutum and Navicula sp. were separated from the source water. Cholorella ellipsoidea, Scenedesmus bijuga, S. quadricauda, Elakatothrix sp. and Chromulina ovalis were separated from the tap water. The blue-green algae from the source water (Nostoc punctiforme, Phormidium tenue, and an undetermined alga) producing toxin or not was uncertain. It needs further study. The algae from the tap water were not reported to produce toxin. At the same time store methodswere studied.4. The algae cell broken methods were primary studied. In the end, it was considered that fresh alage disoved in 40% methanol disposed by ultrasonic is the better method of coarse extraction.