Mechanism Of The Action Between The Severe Acute Respiratory Syndrome-Coronavirus S Protein And The Receptor--Angiotensin-converting Enzyme 2 In Eyes

Objectives: In the present study, we researched the mechanism of the action between the severe acute respiratory syndrome coronavirus (SARS-CoV) S protein and the receptor—angiotensin -converting enzyme 2 (ACE2) in eyes. Methods: PS240-EGFP and pS666-EGFP fusion gene were constructed and were transfected into 293T cells; Expression of ACE2 in human cornea and conjunctiva tissues and cultured corneal and conjunctival cells in vitro was detected bv RT-PCR and immunohistochemistry; The binding capability of the spike proteins of SARS-CoV with cells (Vero E6 cells, conjunctival epithelial cells, conjunctival fibroblast and corneal epithelial cells) and tissues (heart, lung, biain, cornea and conjunctiva) were identified by...