| Objective: The aim of the study is to investigate the effect of exogenous GDNF on spinal cord motoneurons after spinal cord injury in adult rats. Methods: The SCI model of acute posterior compressed spinal cord was established in rats according to the method of Nystrom. The recombinant rat GDNF was injected into the injured region upon intrathecal tubulization in first week after SCI. We observed the remaining motoneurons in anterior horn and the changes of cholinesterase (CHE) and acid phosphatase (ACP) activity by using Nissl and enzyme histochemistry staining. Results: The number of motoneurons in anterior horn in GDNF group was more than that in control group at seven days to four weeks after SCI (p<0.01). The results also shown that GDNF reduced the changes of CHE and ACP activity in lesion cord. Conclusion: These results suggest that exogenous GDNF may protect motoneurons from death induced by incompleted spinal cord injury.
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