| The ovarian cancer is one of the most common gynecology malignant tumors, lacking the valid earlier diagnostic method up to now, the ovarian cancer histological type is complicated , when diagnosed the ovarian cancer , the patient is at late period , the ovarian cancer has a bad prognosis, it has the tallest death rate in the gynecology malignant tumor. The recently research confirm that apoptosis has a close relation to the occurrence , development extinction of the ovarian cancer. Apoptosis is an necessary basic biological process in order to eliminate the latent harmful cell. The cell' s coordination between propagation, differentiation and apoptosis maintains the growth balance of normal tissue. If the apoptosis is not enough or is suppressed, the body can't resume to regulate, express overgrowth and form tumor at last . The colonia of tumor cell needs to carry out the cancer cell's anti-apoptosis test for the maintenance of whole hyperplasy. Reserve the cell which can resist apoptosis and survive and change into cancer cell.That is to say.The tumor cell's high propagation can obtain from the tumor cell' s hyperplasy and poorlydifferentiation , and also from the cancer cell' s apoptosis repressed and existence extension. Currently the people pay more attention to the research of malignant tumor and the apoptosis. The research is increasingly deeper, the FADD can induce the occurrence of apoptosis in various cells line, vivo and vitro binding experiment certificate FADD can specific bind with cytoplasmic domain of FAS, so the FADD play an important part in apoptosis information transmitting process. FADD overexpression can lead the apoptosis, moreover, it is indispensable in FAS, TNFR1 and DR3 mediated apoptosis process. Its important function of FADD is displayed in apoptosis signal conducting path, and also in T-cell generation embryonic development and the application of gene therapy in further research finding . We study FADD expression in ovarian cancer, discuss its function and clinical significance in occurrence and development of ovarian cancer, judge malignant degree and estimate prognosis. Because the FADD induce apoptosis, this study provides new way for the ovarian cancer gene therapy. The expression of FADD were detected in immunohistochemical method in 62 cases of ovarian cancer, 15 cases of benigntumor and 16 cases of normal tissue. All the specimens of ovarian cancer were selected from the tissues without radiotherapy, chemotherapy and macroscopic necrosis. Including serous cystadenocarcinoma 44 cases, mucous cystadenocarcinoma 8 cases malignant teratoma 2 cases. According to the Staging of FIGO in 1985 : stage â… 10 cases, stage â…¡ 16 cases , stage â…¢ 34 cases, stage â…£ 2cases . On the basis of WHO Class well-differentiation 20 cases, moderately differentiation 4 cases, poorly differentiation 38 cases .To analyze clinical pathological data of ovary cancer. The following results through experiments: 1. Positive expression rate of FADD in normal ovarian tissue is 87. 5%. That in ovarian benign tumor is 73. 3% . that in ovarian cancer is 32. 3%. There is significance in the positive expression rate between normal ovarian tissue and ovarian cancer (x 2 =19.17,P<0. 01) . It is also significant between ovarian benign tumor and ovarian cancer (x2=9. 62, P<0. 01) .While it is not significant between normal ovarian tissue and ovarian benign tumor (x2=0. 38, P=>0. 05) . 2. Each specimen positive expression rate is low. regard weak positive as principle, 36 cases , middle positive llcases , strongpositive 0 case. The correlation analysis of expression degree can' t be got, so to analyze every FADD positive expression rate. 3. There is obvious difference of FADD positive expression rates in four FIGO stage (x2 =9. 394, P<0. 05) . In addition FADD positive expression rate is negative to clinical analysis (r=-0.523, P<0.001). There is significance in statistics. 4. The rates in moderately differentiation and poorly differentiation are evidently lower than that in well-differentiation. The difference is significant. 5. The positive rate in serous cystadenocarcinoma is 31.8 %, that in non- serous cystadenocarcinoma is 33. 3% . the difference between them isn't evident (x2=0. 007, P>0. 05) , it is not significance in statistics . In conclusion, the FADD positive expression rates in normal ovarian tissue and ovarian benign tumor are high, that in ovarian cancer is low. That imply: FADD play an important part in the occurrence and development of ovarian cancer . The more later in clinical stage, the more poorer in cell differentiation, the FADD positie expression is more lower , the positive rate is negative correlation to clinical stage. We judge malignant degree of the tumor... |
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