Detection Of Streptomycin Resistant Gene Of Mycobacterium Tuberculosis By PCR And Single-Strand Conformation Polymorphism Analysis

Recently, the emergence of strains of Mycobacterium tuberculosis displaying resistance to some or all of the major anti-tuberculosis drugs has led to renewed interest in streptomycin. It is conceivable that this antibiotic association with other compounds could play a valuable role in controlling tuberculosis. Streptomycin, the first antibiotic used in tuberculosis control programs, percurbs protein synthesis at the ribosome level. It is shown that streptomycin resistance in some clinical isolates of MTB is associated with missense mutations in the rpsL gene .which encodes ribosomal protein S12. In this experiment, 55 sputum specimens of TB patients were gathered .the routine drug susceptibility test was done .meanwhile the TB rpsL gene was amplicated by PCR and analyzed by SSCP. There are 17 strains sensitive to SM and 38 strains resistant to it in routine drug susceptibility test. The SSCP result displayed that 29 specimens were rpsL wild type and 26 strains were mutant type. Compared with routine drug susceptibility test, the specificity was 94.1% and the sensitivity was 65.8%. The result suggested that the rpsL gene mutation was frequently observed in MTB streptomycin resistant isolates and PCR-SSCP might become a simple,rapid and reliable diagnostic test for drug resistance in clinics.