Study On The Determination And Speciation Analysis Of Trace Elements In Chinese Traditional Medicine

Chapter 1: The studies of determination and speciation analysis of trace elements in Chinese herbal medicine were reviewed in both domestic and abroad field. The meaning and content of this research work were represented in this Chapter.Chapter 2: The contents of Cu, Zn, Fe, Mg and Ca in 7 kinds of Chinese herbal medicines, which were planted in the same area, have been determined by flames atomic absorption spectrometry(FAAS). The recovery ratios by standard addition were in the range of 97.6-104.9% and relative standard deviation (RSD) were less than 1.0%. The results of this paper provide a useful data for discussing the relationship between the contents of these elements and the medical effects of these herbal medicines. The results also show that these elements have relativity with pharmacological function of the Chinese herbal medicine.Chapter 3: The root, stem and leaf of Salvia Miltiorrhiza Bye were digested with HNO3-HC1O4(4:1) and the contents of five metallic elements such as K, Cu, Zn, Fe and Mg, which are necessary to humans, were detected respectively by FAAS. The content ratio of Zn to Cu was calculated. The correlation coefficients of different parts and elements were measured by SPSS (Statistical Package for the Social Sciences). It was founded that the contents sequence of metallic elements is as follow: K>Mg>Fe>Zn>Cu. K, Fe, Zn and Cu in leaf were clearly richer than that in the root and stem. The content ratio of Zn to Cu is relatively lower than that in the terrestrial angiosperms. The correlation coefficients between root and stem, root and leaf, stem and leaf of Salvia Miltiorrhiza Bye are 0.998, 0.997 and 0.999 respectively, that means they all have significant correlationships. The correlation coefficient between Cu and Zn was 0.977, which was the biggest among these elements. The results of this paper provide a useful data for discussing the relationship between the contents of these elements in Chinesemedicinal herb Radix Salvia Miltiorrhizae and the medical effects. Furthermore it also provided a data for the distribution of these elements in different parts of Salvia Miltiorrhiza Bye.Chapter4: The contents of trace elements in Saposhnikovia dibaricta(Turcz.) Scischk and its planting soil were detected by FAAS has been presented. Enrichment coefficients of Cu, Zn, Mg, Fe in Saposhnikovia dibaricta(Turcz.) Scischk were calculated. The correlation coefficients between Saposhnikovia dibaricta(Turcz.) Scischk and its planting soil were also measured by SPSS(Statistical Package for the Social Sciences). The results show there are rich elements such as Cu, Zn, Mg, Fe in Saposhnikovia dibaricta(Turcz.) Scischk and its planting soil. The enrichment coefficients of Saposhnikovia dibaricta(Turcz.) Scischk to Cu, Zn, Mg, Fe were in the range of 0.0092—2.190. The enrichment ability of the stem to Cu was the strongest. The correlation coefficients between root and stem, stem and soil, root and soil are 0.970, 0.603 and 0.397, respectively. The results offered a useful data for discussing the relationship between the contents of these elements in Saposhnikovia Dibaricta(Turcz.) Scischk and its planting soil.Chapter 5: Speciation of six elements (Cu, Zn, Fe, Mg ,Ca and Cr) in Radix Astragali was studied. A further more extraction which was based on traditional analytical flowchart program was investigated and distributions of speciation such as extractive rate, residual rate, immerse-residue ratio etc. were also calculated. Water-soluble state and suspension state were separated by micro porous filtering film and adsorption ratio of suspension state was counted. Water-soluble state was divided into organic state and inorganic state based on macro porous adsorption resin and the ratio of organic state to inorganic state was figured out. Further more, organic state and inorganic state in residue were extracted out by non-polar solvent and polar solvent. The study shows that the total extraction rate of the trace elements were high and that means the part which we take was rich in trace elements. The more extraction times, the lower extraction rate. However, the concentrations of the...