Studies On The Pathologic Characteristics And Mechanism Of Severe Acute Respiratory Syndrome Pulmonary Fibrosis

Objective: SARS is a emerged infection characterized fever and pneumonia, case fatality 9.3%, pulmonary fibrosis in late-anaphase. we research the pathologic characteristics and mechanism of SARS pulmonary fibrosis by SARS death patient and macaque model in organ, tissue, cell, deuto-cell, molecular level.Materials and methods: The lung preparation of 6 SARS death patient (died in 9,14,20,29,33,38d) and 6 macaque model (killed in 7,12,14,14, 32, 35d)were objects. Pathological changes, collagen fibers, lattice fibers, elastic fibers, collagen I and III in lungs and fine structure changes were studied by routine H.E dyeing, trigeminy dyeing, trinitrophenol- sirius red staining and polarization microscope, electron microscope and image analysis. Expression of Vimentin, TGFβ₁, TNF α , IL-1β and MMP-2 were detected by immunohistochemistry.Results:1. Pathological changes of SARS death patient. Basic processes of SARS pulmonary fibrosis can be 4 types: fibroblast light degree active stage (in 9-14d), fibroblast proliferation stage (in 20d), fiber proliferation stage (in 29d), fibrosis emerge stage (in 33-38d). collagen fibers is major in fiber proliferation. collagen â…  is obviously to collagen â…¢. Express of Vimentin is increased with time increase, in 20-38d significant deviation or highly significant deviation compared to control (p<0. 05, p<0.01). In early stage the express of TGF- β₁ light increase, in 29-38d significant deviation or highly significant deviation compared to control(p<0.05, p<0.01). Express of IL-1 β and TNF- α significant increasein in 9d, to peak time extreme strong masccline in 14d,...