| OBJECTIVE:By the animal experiments, to discuss the different effects of hyperthermia, chemotherapy, radiotheraphy, chemohyperthermia, Radiohyperthermia, and radiochemohyperthermia on the expression of colon cancer cell's E-Cad, β-cat, P53, Bcl-2, and Bax; and to reveal the booster mechanism in radiotherapy and chemotherapy of hyperthermia. METHODS:Human colon cancer cells (HT29) were transplanted into the behind limbs of the naked mice. Under the laboratory simulated conditions of hyperthermia ( 4 3℃ , 6 0 min) ,referring to the clinical chemotherapy prescription for human colon cancer (MMC), the actual doses were calculated, and referring to the clinical radiotherapy prescription for human colon cancer, the actual radio doses were calculated. In this experiment, the mice were divided into 6 groups: hyperthermia group, chemotherapy group, radiotherapy group, chemohyperthermia group, radiohyperthermia group, and radiochemohyperthermia group. The mice were sacrificed and the tumor tissue was taken at different time points( before treatment, at 2h, 4h, 8h, 12h, 24h, 48h, and 72h after treatment). The morphologic changes in tumor cell memberance, plasm and nucleus evoked by the expression E-Cad, β -Cat, P53, Bcl-2, and Bax were observed by the method of immunohistochemistry stain(S-P method). RESULTS:1. The expression of E-cad could be upregulated separately by hyperthermia, chemotherapy, radiotherapy, chemohyperthermia, Radiohyperthermia, and radiochemohyperthermia; and the allied therapy of hyperthermia, chemotherapy, and/or radiotherapy made this upregulation more prominent than any singletherapy. The dynamic analysis revealed : E-cad increased gradually in 12h~72h after hyperthermia. E-cad showed an instant but gradually increasing tendency in 8h~24h after chemotherapy. E-cad increased gradually in 24h~72h after radiotherapy. It also increased gradually in 8h~72h after chemohyperthermia as well as radiohyperthermia . It showed a gradually increasing tendency in 4h~72h after radiochemohyperthermia (colour picture 1) .2. None of hyperthermia, chemotherapy, radiotherapy, chemohyperthermia, Radiohyperthermia or radiochemohyperthermia made an obvious effect on P -Cat. The dynamic analysis revealed that the expression of ￡ -Cat showed no significant difference among each group at all selected time points (colour picture 2).3. The expression of P53 could be downregulated separately by hyperthermia,chemotherapy, radiotherapy, chemohyperthermia, Radiohyperthermia, and radiochemohyperthermia. And the downregulating effect of radiochemohyperthermia was the most significant. The downregulating effects on the expression of P53 shows no significant difference among the hyperthermia group, the chemohyperthermia group, and the Radiohyperthermia group. However, each of these 3 groups downregulated the expression of p53 more significantly than either of chemotherapy group and radiotherapy group. The chemotherapy group downer-gulated the expression of p53 more significantly compared with the radiotherapy group. The dynamic analysis revealed :the expression of p53 showed a gradually lowering tendency in 2h~72h after hyperthermia, in 4h~72h after chemotherapy as well as in 12h~72h after radiotherapy. In 2h~48h after chemohyperthermia the expression of p53 lowered gradually, while it showed a rising tendency in 48h~72h. In 2h~48h after radiohyperthermia the expression of p53 showed a gradually lowering tendency. In 2h~48h after radiochemohyperthermia the expression of p53 lowered gradually till 48h when the mean optimal density was 0.16, yet it showed an ascending tendency in 48h~72h(colour picture 3).4. The expression of Bcl-2 could be downregulated separately by chemotherapy,-5-radiotherapy, chemohyperthermia, Radiohyperthermia, and radiochemo-hyperthermia. And the allied therapy of hyperthermia, chemotherapy, and/or radiotherapy made this downregulation more prominent than any single therapy. Hyperthermia seemed to have no significant effect on the expression of Bcl-2. The dynamic analysis revealed : In 4h~8h after hyperthermia the expression of Bcl-2 lowered slightly transitorily , then rebounded to the level as before the treatment, and such status maintained to 72h, but the expression of Bcl-2 showed no significant difference at each selected time point. After chemotherapy the expression of Bcl-2 showed a gradually lowering tendency in 8h~48h, then it showed a rising tendency in 48h~72h. The expression of Bcl-2 showed a gradually lowering tendency in 8h~48h after chemohyperthermia, then it showed a slightly rising tendency in 48h~72h. After Radiohyperthermia the expression of Bcl-2 showed a gradually lowering tendency in 4h~48h, then it showed a rising tendency in 48h~72h. After radiochemohyperthermia the expression of Bcl-2 showed a gradually lowering tendency in 2h~48h, then it showed a rising tendency in 48h~72h(colour picture 4).5. The expression of Bax was upregulated separately by hyperthermia, chemotherapy, radiotherapy, chemohyperthermia, Radiohyperthermia, radiochemohyperthermia; and the allied therapy of hyperthermia, chemotherapy, and/or radiotherapy made this upregulation more prominent than any single therapy. The dynamic analysis revealed : the expression of Bax increased gradually in 24h~72h after hyperthermia .After chemotherapy, it also increased gradually in 8h~48h and peaked at 48h .And the high level maintained till 72h. After either of radiotherapy and chemohyperthermia, it also increased gradually in 8h~48h, peaked at 48h and maintained till 72h. After Radiohyperthermia, it also showed a gradually increasing tendency in 4h~48h. It peaked at 48h and maintained till 72h. After radiochemohyperthermia , it showed a gradually increasing tendency in 2h~48h, then showed a lowering tendency in...
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