The Study Of MRNA Of Ilterleukin-1alpha,Beta And Interferon-gamma In Macrophages In Endometriosis: Comparison Of Eutopic And Ectopic Endometrium With Normal Endometrium

OBJECTIVES: Immunologic mechanisms are implicated in the pathogenesis of endometriosis. The purpose of this study was to assess IL-1 alpha, IL-1 beta and IFN-gamma mRNA in macrophages in eutopic and ectopic endometrium of endometriosis women and to explore the role and activity of macrophages in endometriosis.METHODS: Forty women with histopathological diagnosed endometriosis after operation at stage I ~IV according to the criteria by Revised American Fertility Society were selected as endometriosis group, eutopic endometrial samples were histologically divided into proliferative (n = 18) and secretory (n = 22) phases.Fifteen women with tubal ligation and reana stomosis of tubes were selected as control group, eutopic endometrial samples were histologically divided into proliferative (n = 8) and secretory (n = 7) phases. Ectopic endometrium obtained at operation from forty women with endometriosis were compared with eutopic endometrium from all women with and without endometriosis. Tissues were frozen in ultra low -85℃ immediately,then formalin-fixed, paraffin-embedded, sectioned and stained using the in situ hybridization technique to assess IL-1 alpha , IL-1 beta and IFN-gamma mRNA in eutopic and ectopic endometrium. Positive control tissues (cervical polyp) and negative controls (no primary probe) were used in each experiment.RESULTS: Expression of interleukin-1 alpha(IL-1 alpha) and interleukin-...