Experimental Study Of The Effect Of Endotoxin On The Open Abdominal Wound With Seawater Immersion And Its Mechanism

Objective To investigate the change of plasma endotoxin level and the effect of lipopolysaccharide-binding protein (LBP), CD14. Methods Sixty male Wistar rats were randomly divided into six groups: normal group, open abdominal wound (control) group, open abdominal wound with seawater immersion 0.5h, 1h, 2h, 3h , groups (n=10). Plasma endotoxin levels were tested. LBP and CD14 proteins were investigated in liver and lungs. Pathological examinations of specimens were performed. Meanwhile, other 36 male Wistar rats were randomly divided into 6 groups to exam the expression of LBP, CD 14 and TLR4 mRNA. Results Morphologic changes of internal organs showed that lesions in the groups of open abdominal wound with seawater immersion were more severe than those in the control group. After 2 hours of seawater...