Experimental Study On The Effect Of Arsenic Trioxide In Inducing Apoptosis And Suppressing Expression Of VEGF On Human Hepatocarcinoma Cell Lines BEL-7402

Objective: To investigate the effect of arsenic trioxide (AS2O3) in inducing apoptosis on human hepatic carcinoma cell line BEL-7402 and the expression of apoptosis-related protein Bcl-2/bax. Then, to illustrate the relationship between the expression of vascular endothelial growth factor (VEGF) and the invasion and metastasis of hepatocellular carcinoma (HCC).and to explore the effect of arsenic trioxide(As2O3) on production of VEGF in human hepatocarcinoma cell lines BEL-7402Method: The cell line (BEL-7402) was treated with various concentrations of As2O3, and then cultured in RPMI-1640 medium for24, 48, 72hours respectively. Thereafter, MTT assay was used to study the cell inhibitory rate; Giemsa staining and acoidine staining were used to show the morphologic change; FCM was employed to analysis the cell cycle kinetics and detect the expression bcl-2 and bax protein. Immunohistochemical staining was taken to reveal the change of the expression of VEGF after treatment.Result: MTT assay showed that AS2O3 can inhibit cell proliferation in a dose and time dependent manner. Morphological study showed that, with the dose of AS2O3 increasing, the cell adhesion ability decreased, many cells shed from the culture bottle wall and floating in culture medium. 72 hours later, about 80% of cells were shed. Even at the lower concentration (1 μ mol/L), AS2O3 also can significantly inhibit the cell proliferation. A typical set of changes such as cell shrinkage, cleavage of nuclear DNA, chromatin condensation, membrane blending and apoptotic body formation will be observed. The cells arrested in G2/M phase were obviously increased, a...