The Reproductive And Developmental Toxicity Of Formaldehyde And It's Mechanisms On Mouse Embryos

Objiective: To observe the reproductive and developmental toxicity and to explorethe possible mechanisms of formaldehyde on mouse embryos in vitro.Methods: In vivo animal experiment: to conduct micronucleus and chromosomeaberration experiments with the fetal liver blood of the mice from the different groups, in order to observe the rate of MN and the rate of chromosome aberration. In vitro experiment: the postimplantation whole embryo culture (WEC) system was used to explore the effects of formaldehyde on cultured embryos in vitro. The 8.5 day isolated kun-Ming mice embryos (3~5 somites) at organogenetic stage were co-cultured for 48 hours in immediately centrifugal serum (ICS) with formaldehyde (final concentration of formaldehyde: 0, 3, 9, 27, 81μmol/L). To explore the characteristic of reproductive and developmental toxicity and their mechanisms of formaldehyde, the following techniqued were used in the study: neutral red staining were used to detect cell death ; flow cytometry were used to analyse the effects of cell cycle and the rate of apoptosis; Light microscope were used to observe damaged on structure of visceral yolk sac (VYS) and cultured embryos; The contents of GSH and MDA in embryo tissue were measured by relative detection kits; When 100μg/ml VitC was added to the culture medium along with formaldehyde, VitC could intervene effectively the developmental toxicity of formaldehyde on mouse embryos in vitro.Results:1. Formaldehyde could penetrate placental barrier and result in a significant impairment on the chromosome in fetal mouse.2. Formaldehyde could cause damage to embryonic development and there were dose-effect relationships and time-effect relationships in vitro. The major abnormalitied included abnormal nervous system (small forbrain and midbrain, open neural tube), heart (retarded heart , pericardial edema), abnormal flexions, small...