The Design On The Experimental System For Hypoxia Cell Culture In Vitro

Exploring the cell's occurrence, development, configuration and function, mechanism of pathological changes, characteristic of consenescence and decease has being the active field in the domain of biomedical research. And As the basic for the whole experimental work, designing the model of hypoxia cell culture is very important. However, organism is a complex system which organizes and accommodates both by itself, so owing to the staggering complexity of the in vivo environment, establishing an experimental system for hypoxia cell culture in vitro proved with acceptable precision is the indispensable experimental method in the biomedical researches.An experimental system for hypoxia cell culture in vitro supported was established in this paper, on the elementary demands of the experiment for hypoxia cell culture. The following results were attained,(1) The thesis reviewed the current literature and summarized the up-to-data experimental devise and methods for hypoxia cell cultured. The merits and demerits of each design were especially analyzed.(2) A devise for hypoxia cell culture was established based on the hardware control design.(3) A measuring and controlling system for hypoxia cell culture established based on the PC control. The modified system can not only process the sampling data more efficiently with the data pretreatment and fixed value control arithmetic, butalso provide an interface for manipulating and observing in real time.(4) Measuring and controlling system Emendation by experimentation considering the sampling delay for the gas diffusing. And acquire the delay coefficient used in optimized arithmetic. After emendation by experimentation, the precision reaches 0.8% in the oxygen consistence controlling range froml% to 5%, and reaches 0.7% above 5%.(5) Aiming at the deficiency of on-off control system, this paper provided a optimized arithmetic, PID Control with the Smith Precompensation, from the continuous control theory. Theoretic preparative was made for further improvement.