Study The DNA Vaccine And Anti-Viral Drugs For Prevent And Therapy Of HCMV Infection And Disease

Human cytomegalovirus is the important etiology infected in fetal intrauterine infection and perinatal stage. And it is also the one important pathogen of some serious disease which caused by immuno-inhibitor used, organs and bone marrow transplantation or AIDS. Many research show that HUMV is more related to some tumor, such as cervical carcinoma, prostate carcinoma. Colon cancer and so on.HCMV gB can express on the surface of virion and infected cell, the Ag epitope which recognesed by specific Ab can induce the immune reaction , so it is the important protein for prevent the disease which caused by HCMV.And now, researches showed that many anti-viral drugs have a better curative effect on some disease caused by HCMV. However, in some long-term treatment patiens, the drug resistance is easy to occure. And we also found that the drug which used to treatment the infection caused by virus have some virulence to body. So ,it is important to develop a new anti-viral drug to therapy the infection of HCMV. I. Constructing HCMV gB gene expression vector as DNA vaccine and detecting its immunityObjective to construct human cytomegalovirus glycoprotein B expression vector pCMV.gB and to evaluate its immunological effects as DNA vaccine in the mouse in-vivo model.Methods we constructed HCMV pCMV.gB expression vector by inserted PCR production gB gene to the plasmid pcDNA3. BALB/C mice were immunized by pCMV.gB by hypodermic injection. Body temperature and weight was measuredevery two weeks .NK cell activity was tested by LDH release test. MTT method was used to detect T cell proliferation activity and IL-2 biological activity. The serum IgM and IgG levels of HCMV were detected using ELISA method. The concentrations of IL-2 and IL-4 in sera and supernatant of spleen cells were detected using ELISA.Results The HCMV DNA vaccine expression vectors pCMV.gB were successfully constructed. The common conditions of mice were well after being immunized. There was no significant difference of weight and body temperature between the immunized mice and the corresponding control. T cell proliferation activity and NK cell activity significantly increased, and specified IgM and IgG for HCMV were induced. The concentrations of IL-2 and IL-4 and activity of IL-2 in supernatant of spleen cells were increased.ConclusionHCMV DNA vaccine of pCMV.gB induced immunological reaction by this approach. II. Study the mechanism of the new anti-viral drug1. To observe the virulence of the drug2. To observe the control effect of the drug3.To examine the mRNA of IE gene and L gene of HCMV by RT-PCR. Conclusion: The study showed that the drug is safety to the culture cell and caninhibit the replication of HCMV.