The Study On Effect Mechanism Of Alpha-terthienyl To Aedes Albopictus Larvae

Objective:To study the toxicity, cross-resistance with deltamethrin and LPO of alpha-terthienyl to Aedes albopictus larvae.Methods:1. To determine the toxicity of alpha-terthienyl and deltamethrin to sensitive and resistant Aedes albopictus 4^th larvae by biologic assay.2. To detect protein content, esterase activity and MDA content of Aedes albopictus 4th larvae by biochemistry method after treated by UVA and alpha-terthienyl.3. To investigate the LPO changes of Aedes albopictus 4^th larvae by histological method.Results:1. Under UVA and alpha-terthienyl, sensitive and resistant Aedes albopictus 4^th larvae showed same toxicosis2. The LC₅₀ (median lethal concentration) was 5.34μg/L and 9.09μg/L respectively for sensitive and resistant group of Aedes albopictus 4^th larvae after treated by UVA and alpha-terthienyl;3. The LC₅₀ (median lethal concentration) was 0.54μg/L and 444.17μg/L respectively for sensitive and resistant group of Aedes albopictus 4^th larvae after treated by deltamethrin;.4. There was no cross-resistance between deltamethrin and alpha-terthienyl.5. Under UVA and alpha-terthienyl, protein contents of resistant and sensitive Aedes albopictus 4^th larvae were all raised and esterase activity were all decreased.6. Under UVA and alpha-terthienyl, MDA content of resistant and sensitive Aedesalbopictus 4th larvae were all raised, the higher dose of alpha-terthienyl, the higher of MDA content.7. The LPO changes were discovered in midgut and malpighian tubule of Aedes albopictus 4th larvae after treated by UVA and alpha-terthienyl.Conclusion:1. Alpha-terthienyl is a light-activated, high effect to kill both sensitive and resistant Aedes albopictus larvae;2. There is no cross-resistance between deltamethrin and alpha-terthienyl;3. Under UVA and alpha-terthienyl, protein contents and MDA content of Aedes albopictus larvae of 4th instar are raised and esterase activity are decreased;4. The LPO changes occur in midgut and malpighian tubule.