A Novel Animal Model For Comparison Of Reducing Mucus Production Of Ileal Conduit After The Treatment Of Different Drugs

Objective To develop a novel canine model for comparison of reducingmucus production of resevoirs after the treatment of different drugs.Methods To design the experiment according with the statisticalprinciple of latin square .The study comprised 6 adult healthy female dogs.Ineach dog , we constructed 6 "Ileal conduits"with a 50cm long ileum .The "ileal conduits"were instilled with anhydrous alcohol ,87.5%alcohol ,2% tinctura io di, 1% tinctura io di, 10 % formalin andplacebo(saline) during surgery .And the conduits was instilled with theartificial urine for 5 hours every day after operation .The "urine"sampleswere analyzed for mucus protein. 2 weeks later, the mucosa samples werecollected and were analyzed after AB-PAS (Alcian Blue-Periodic acid Schiff)assay .And the morphological changes were observed through microscopeand electron microscope. The mucus secretion, goblet cell count , height ofvilli and crypt depth et al were studied.Results The canine model wsa established successfully in all 6 dogs. Thedata comfirmed that the use of active treatment in our study did result in asignicant reduction in mucin production.And the changes of histology wereobvious (p<0.005).Conclusion The anhydrous alcohol has advantages of reducing mucinthan the other drugs . The model is proved to be successful .