| Objective: Through developing observing the change of motor action, weinvestigate the effects of ephedrine on motor recovery after middle cerebralartery occlusion in rats, and explore the molecular mechanism of ephedrinein accelerating rehabilitation by detecting the expression of GAP-43 andSYP. Draw a conclusion to direct treating cerebral infarction. Methods: Along with the application of intra-luminal technology of modifiedLonga, fishing-line was used to create the focal cerebral ischemiareperfusion model in Clean-Grade Sprague-Dawley rats, extract the lineafter occlusion 2h. The rats were randomly divided into three groups:sham-operated group, natural recovery group and ephedrine treatmentgroup. Beam walking test was used to evaluate the improvement of motorfunction at week 1, 2, 3 and 4 after operation. The ultra-structure changesof ischemic tissues were observed by transmission electron microscope.The expression intensity of GAP-43 and SYP around ischemia area wereexamined by immunohistochemical techniques. The gene expression ofGAP-43 and SYP were observed by semi-quantitative RT-PCR at oneweek. Results: (1) The beam walking test shows the ephedrine treatment grouprecovery faster than the natural recovery group. (2) The immunoreaction products of GAP-43 reach the peak at 1week, degrade at 2 weeks. The natural recovery group has not significancecompared with sham-operated group at 4 weeks(P>0.05). Ephedrinetreatment group demonstrated statistically increases in GAP - 43 asdetermined by optical density measurements compared with naturalrecovery group at 1, 2 and 3 weeks(p<0.05). (3) The immunoreaction products of SYP reaches the peak at 2 weeks.The density measurements of ephedrine treatment group and naturalrecovery group demonstrated statistically significant increases comparedwith sham-operated group at all time points. Ephedrine treatment groupdemonstrated statistically increases in SYP as determined by opticaldensity measurements compared with natural recovery group at 1, 2 and 3weeks(p<0.05) (4) The expression level of GAP - 43 and SYP genes mRNAsignificant increase in ephedrine treatment group, which has significancecompared with natural recovery group. Conclusions: Treatment with ephedrine may facilitate recovery from behavioraldysfunction following brain injury in rats. ephedrine facilitate theexpression of GAP-43 and SYP both in protein level and in gene level.One of the mechanisms of behavioral recovery with ephedrine treatment isassociated with the enhanced expression of molecules involved in neuronalremodeling.
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