Expression Of HMGB1 In Spleen In MODS Mice

At present, uncontrolled system inflammation has become the most current etiological explanation of multiple organs dysfunction syndrome (MODS). Recent researches showed that high mobility group box-1 protein (HMGB1) is a crucial late mediator of lethal systemic inflammation induced by sepsis or endotoxin and likely to be a potential efficient therapeutic intervention point to MODS. To find out whether HMGB 1 is concerned with the development of MODS induced by different causes and how HMGB1 express and release during the course, to comprehend the function of immunity organs in release of HMGB 1 and the effect of HMGB 1 in immunity function, BALB/c mice models for MODS were replicated by intraperitoneal injection of zymosan, then the change of HMGB1 serum concentration was measured and the expression of HMGB1 in spleen was observed. Simultaneously, the changes of spleen pathology, some immunity-related measures and functional state of spleen dendritic cells (DCs) were observed following zymosan injection.We found that the serum HMGB1 level ascended 12h following zymosan injection and reached to the peak 1d later then descended and approached the level before injury. 10d after zymosan injection, the serum HMGB1 level ascended markedly again. Expression of HMGB1 in spleens increased earlier, which increased 3h after injury and reached a low peak 5h later. Following a vale, another expression peak, far higher than the former, occurred 2d after injury. Expression of HMGB1 in spleens decreased and returned near to normal 5d later, but increased slightly 10d following injury. We also found cell apoptosis in spleens during the course of...