Association Of Oestradiol, Progesterone Concentration And The Expression Of Nuclear Factor-κB In Peripheral Blood Mononuclear Cells In IVF/ICSI Cycles And IVF/ICSI Outcome

Part IObjective: To investigate whether serum oestradiol and progesterone levels in the different time of IVF/ICSI cycles affect IVF/ICSI outcome. Methods: 170 women underwent 170 IVF/ICSI cycles were prospectively analysed. They were employed contrail oocytes hyperstimulation (COH) with a long protocol of gonadotrophin-releasing hormone agonist (GnRH-a) / follicle-stimulating hormone (FSH) or (and) human menopausal gonadotrophin (HMG) / human chorionic gonadotrophin (HCG). Serum oestradiol and progesterone concentration on the day of HCG administration and the day of embryo implantation (ET) and the 14th day after ET were measured by chemiluminescence immunoassayed. Results: (1) Biochemical pregnancy rates (BPRs) were the highest when oestradiol levels were in 8000~20000pmol/L group, however, high P levels( >9.54 nmol/L) on the day of HCG administration were associated with lower BPRs (P<0.05) . (2) On the day of ET, higher E2 leveles were with higher BPRs but no statistics significance; significantly lower BPRs were recorded in the cycles which P levels <400nmol/L. (3) The ratio of oestradiol concentration on the day of HCG administration to the day of ET of oestradiol concentration ( HCG-E2/ET-E2 ) was highly predictive of IVF/ICSI outcome: the ongoing BPRs were 26.15% respectively if the ratio higher than 3.5, compared to 46.67% if the ratio lower than 3.5. (4) Higher E2 leveles on the day of HCG administration, higher E2 and P leveles on the day of ET, and higher E2 leveles...