| Panax notoginseng is highly prized in China for its therapeutic abilities to stop haemorrhages, to influence blood circulation and to act as a tonic agent. Saponins (PNS), the major bioactive components in Panax notoginseg, are used to treat coronary heart disease, cardiac angina, apoplexy and atherosclerosis in clinic. These effects are correlated with their anti-platelet aggregation. However, PNS contain several kinds of active components such as notoginsenoside R1, ginsenoside Rg1, Rb1, Rc, Rd and Rf. It is still unknown that which saponin is mainly contribute to the therapeutic effects of PNS.Modern pharmacological studies have shown that combination with some receptors or channels on cell membrane is the first step of drug action. In this article, platelet bio-specific extraction coupled with high performance liquid chromatography (HPLC) was used to screen anti-platelet aggregation agents in PNS, which can eliminate the interference of lots of non-bioactive agents, and have the characteristic of high efficiency and high selectivity. In brief, first, HPLC fingerprint of PNS was developed. Secondly, PNS was added into platelet and interaction was performed at physiological conditions. Then, no combined components were removed by washing with phosphate buffered saline. Finally, changed the elution buffer pH to destroy the platelet, the bound components would be delivered. The eluent was collected for HPLC analysis. Comparing the HPLC profiles of eluent and PNS, we easily found the components which combined with platelets (potential anti-platelet aggregation agents). Furthermore, we confirmed their biological activity by using pharmacological assay. Based on the research above, we found that Saponin B, A and C are the main anti-platelet aggregation components in PNS.
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