| Objective To study the antiarrhythmia action of Ginsenoside Re(GSRe) and its effect on cardiac function and hemodynamics, histochemistry of myocardium and cellular ultrastructure.Methods Thirty-six rabbits were randomly divided into six groups. Each group had six rabbits. Isoproterenol(ISO) was dissolved with 0. 9%NaCL, and then rabbits were injected with it by the speed of 5mg/kg min. The speed was controlled by transfusion pump. After about 30 minutes, Ventricular tachycardia(VT) was appeared. At the same time, GSRe was dissolved with 1, 2-propanediol (1, 2-PRO). When the VT was appeared, rabbits were injected with GSRe or Verapami 1 (VER) or equate menstruum. In small dose GSRe treated group, the dose was 5mg/kg. In middling dose GSRe treated group, the dose was 10mg/kg. In big dose GSRetreated group, the dose was 20mg/kg. In the Verapamil treated group, the dose was 0. 4mg/kg. In the blank group, equate menstruum was injected. In the VT model group, equate menstruum was injected. And then ISO was injected in those rabbits with a constant speed 5mg/kg.min. Each rabbit was inserted with 4F catheter in dexter femoral and left carotid. And then, we mensurated the hemodynamics of the rabbit with AgilentM 1165 /66/67/75/77A whole function wardship system and we observed the variety of electrocardiograph with AgilentM120AV24/V26 heart-electricity module.They were left ventricle systolic pressure (LVSP),left ventricle end diastolic pressure. (LVEDP),mean arterial pressure(MAP), heart rate(HR). With the data, we calculated cardiac muscle oxygen use(CMOU). When rabbits were injected with ISO for 1 hour, rabbits were executed. And then, cardiac muscle in the cuspidate position of left ventricle were chose to check with optical microscope and electron microscope. Through the animal model of ventricular arrhythmia, we study the effects of GSRe on recovery of myocardial function and hemodynamics, histochemistry of myocardium and cellular ultrastructure.Results 1. By the speed of 5mg/kg.min, ISO could make triggered ventricular arrhythmia rabbit model in vivo. Compared with the normal group, HR, LVSP, LVEDP, MAP and CMOU in the ISO model group showed markedness difference (P<0. 01).2. Verapamil could treat the triggered ventricular arrhythmia rabbits by the speed of 0. 4mg/kg. The time which keep sinus rhythm were 177. 00 + 5. 66s. Compared with the ISO model group, HR, LVSP, LVEDP, MAP and CMOU of 1, 3, 5minutes in the verapamil group showed markedness difference (P<0. 05).3. GSRe could treat the triggered ventricular arrhythmia rabbits in vivo. In the big dose group, the time which keep sinus rhythm was 177. 83 ±5. 31s. In the middling group, the time which keep sinus rhythm was 21. 00 + 2. 83s. In the small dose group, the time which keep sinus rhythm was 4. 50+1. 64s. Compared with the ISO model group, HR, LVSP, LVEDP, MAP and CMOU of 1,3,5 minutes in the three GSRe treated group showed markedness difference(/<0. 05). It showed dose-depended relation. The value of rs was between -0. 971 and -0. 408 (KQ. 05). Hemodynamics of the big dose GSRe treated group were resembled with the Verapamil treated group.4. Cardiac muscle optical microscope checked results showed that the blank treated group was gave priority to zero grade. The big and middling GSRe treated group were gave priority to zero and 1 grade. The model group was gave priority to 3 and 2 grade. Compared with the model group, the big dose and middling GSRe treated group showed markedness difference(K0.05). The small dose GSRe treated group showed no difference(/>0.05). Cardiac muscle electron microscope checked results showed that the structure of the blank treated group were normal. In the model group, we could see microsome swelled, endoplasm enlarged, muscle fibres arranged wrongly, and even muscle membrane were in rupture. Some nucleolus chromatins were collected in the side. These caused the conformation changes of apoptosis. In the GSRe treated group, the changes of apoptosis were fewer.Conclusion 1. ISO could make triggered ventricular arrhythmia rabbit model in vivo.2. GSRe could reduce left ventricle systolic pressure,left ventricle end diastolic pressure, heart rate, mean arterial pressure...
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