The Study Of Express And Correlate Factor Of Tape â…  And â…¢ Precollagen MRNA In Human Atria Of Rheumatic Heart Disease

Object: Rheumatic heart disease induces global change in myocardial architec-ture,called post- rheumatic heart disease myocardial remodeling,which involves myocardial dilation myocyte hypertrophy, and in terstitial fibrosis ,leading to a deterioration in heart function that is asso-ciated with increased human of mortality and deformity.Rein- angiotensin system (RAS) especial angiotensin II (AngII)plays an important role inthis process, the purpose of this study was to determine whether atrial expression of the collagen I, collagen III and the (AT₁ , AT₂) is altered in patients with rheumatic heart disease and atrial fibrillation (AF) or Chronic heart failure.Methods: A total of 40 patients with rheumatic heart disease were included thirteen patients had no history of AF(group A) ,10 patients had paroxysmal AF (group B) and 17 had chronic persistent AF (≥6 months ) as group C ,and 8 normal as group E Atrial tissue was obtained from the right atrial appendage during valve replacement surgeryl Total RNA was isolated and reversely transcribed into cDNA in a semi-quantitative polymerase chain reaction the cDNA of interest genes and the housekeeping gene GAPDH were coamplified and separated on an agarose gel by electrophoresis and stained with ethidium bromidel The levels of mRNA expression were quantified by densitometryo The activities of cardiac muscle angiotension II were examined by the method of radioimmunoassay test.Result: The amount of collagen I /GAPDH was increased to 0.58 in group A ( P = 0.001) and to 0.91 in group B( P < 0.05) and to 1.21 in group C( P <...