Inhibitory Growth Effect Of Recombinant Apoptin Eukaryotic Expression Plasmid On Humane Ovarian Cancer Xenografts In Nude Mouse Model

Objective To investigate the inhibitory growth effect of recombinantapoptin eukaryotic expreesion plasmid on human ovarian cancer xenografts in nude mouse modle.Methods The recombinant eukaryotic expression plasmid pcDNA-VP3 wassuccessfully constructed. Approximate 2x10~6 cells of ovarian cancer cells (COCl) were implanted subcutaneously into per nude mouse. Twelve female Balb/c nude mice were divided into as follows four groups: High doesage group and low doseage group were received intratumoral injection at does of 100 μg and 50 μg pcDNA-VP3 plasmid cDNA respectively.Plasmid control group and lipofectamine control group were received injection at does of 100 μg pcDNA3.1(+) cDNA and the same volume of liprofectamine.The length and wideth of the xenograft tumor were mearsured every threee days, caculate the volume of the tumor according to the formula:V=LxW2x0.52. These mice were sacrificed after twelve days,and their tumors were excised .The mean tumor weight per group were caculated.RT-PCR was used to detect mRNAexpression of VP3 gene .Apoptosis of the xenograft tumor was observed by TUNLE assay analysis.Resu I ts (1) Recombinant eukaryotic expression plasmid pcDNA-VP3 wasconstructed successfully.(2) The tumor growth inhibitory rate of high dose of pcDNA- VP3 group was76%(p<0.05).The tumor growth inhibitory rate of low dose of...