The Study Of P27 And Skp2 In The Juman Gastric Carcinoma

Objective:By examining the expression of the cyclin-dependent kinase inhibitor p27 and S-phase kinase-associated protein 2(Skp2) through mRNA and protein levels in human gastric carcinoma(GC), analyzed the correlationship between Skp2 gene and p27 gene; investigated the clinical and biological significance of their expression in the human GC. Methods:We measured the Skp2 and p27 mRNA level and protein level expression by reverse transcription polymerase chain reaction (RT-PCR) and SP immunohistochemical method in 42 GC specimens and 42 nomal gastric mucosa. Result:(1)By RT-PCR method, we found that the GC tissue (0. 6767 + 0. 1683) showed no significant higher level of p27 mRNA expression compared with normal gastric mucosa(0.6944 + 0.1866)(t=-0. 456 P=0. 65); while the Skp2 mRNA expression was 0. 2791 ±0. 1829 and 0. 7691 ±0. 2514 in the GC tissue and normal gastric mucosa, which showed a stastic significance(t=—10.212 P=0. 0009). Regard to the clinical pathology characters, we found the Skp2 mRNA expression showed a higher level in patients with larger tumor diameter, poorer differentiation, lymph nodal metastases positive, serosal invasion. The Skp2 mRNA expression rate was 0.8675 ± 0. 1120 and 0.4543 10.3169 in low differation and high-middle differation GC tissue, which showed the stastic difference (t=-4. 045, P=0. 002). The Skp2 mRNA expression in tumor larger than or equal 5 cm(0. 8460 + 0. 2051) was significantly higher than that in smaller tumors (0. 6154 + 0. 2719; t=-3. 077, P=0. 004). Moreover Skp2 mRNA expression was more frequent in lymph nodal metastases positive ones (0. 8507 + 0. 2074) compared with lymph nodal metastases negative ones (0. 6222 + 0. 2633, t=-3. 104 P=0. 003).