Study On Epidemiology Of Cryptosporidium Parvum Infection In Anhui Province And Expression Of Cellular Immune Function In Persons Infected By Cryptosporidium Parvum

Objective To provide scientific evidence for prevention and controlling of the infection of Cryptosporidium parvum and its epidemiological characteristics were studied in some areas of Anhui Province. To explore the changes of cellular immune function in the persons infected by Cryptosporidium parvum.Methods The oocyst of Cryptosporidium parvum in 5421 fresh stool samples from eleven areas of Anhui province was tested by auramine-phenol stain and auramine-phenol stain and improved anti-acid stain respectively. T subsets, membrane interleukin-2 receptor (mIL-2R) in peripheral blood mononuclear cell (PBMC) and soluble membrane interleukin-2 receptor (sIL-2R), interleukin-6(IL-6), soluble intercellular adhesion molecules-l(sICAM-1) in serum of the persons infected by Cryptosporidium parvum were detected by the system of Biotin-Streptavidin (BSA) and ELISA respectively.Results The total infective rate of Cryptosporidium parvum was 1.22% (66/5421) in Anhui Province. Among them, the positive rates of oocyst in the areas of Huaibei[1.82%(8/440)]and Fuyang[1.80%(9/500)]were higher than those of other areas. The positive rates of oocyst in stools of infants, pupils, middle school students, college students, adults were 3.15%(28/889), 0.82%(9/1098), 0.82%(9/1092), 0.83 %(8/969) and 0.87%(12/1373) respectively. And among them, the positive rate of oocyst in stools of patients with diarrhea was 2.88% (8/278), and the persons with immunodeficiency was 8.33% (3/36). The positive rates of oocyst in infants and the patients with diarrhea and immunodeficiency were significantly higher than those in others (P<0.01). The positive rate of oocyst in males was similar to that in females (P>0.05). The positive rate of oocyst in urban areas[0.98 % (32/3276)] was significantly lower than that in rural areas[1.59%(34/2145)](P<0.01). The main manifestations of the patients were subclinical infection, in forms of slight abdominal pain, mild diarrhea, and loose stool. The positive rates of CD3~+, CD4~+, CD8~+ and CD4~+/CD8~+ in stools of persons with positive of Cryptosporidium parvum were (54.77±7.33)%, (38.17±7.52)%, (30.64±5.87)% and (1.28±0.72)% respectively, and the level of mIL-2R in silence and induction was (2.91±1.23)% and (32.15±2.42)%. The levels of CD3~+, CD4~+, mIL-2R and the ratio of CD4 ~+ / CD8~+ between the persons with positive and negative oocyst were significant different (PO.01~0.05). The levels of sIL-2R, IL-6 and sICAM-1 in serum of the persons in whose stools Cryptosporidium parvum oocyst was found were (588.3± 214.2) (xg/ml,...