Autofluorescence Research Of Cardiac Cancer And Normal Gastric Corpus

Background and ObjectiveLaser induced autofluorescence (LIAF) technology applied in the diagnosis of tumor has been developing since the late 1970s'. LIAF can display the information inside the live human tissue constitution, and avoid some side effects caused by injecting fluorescence reagents that was noticed by researchers from different countries immediately. And the rapid development of modern laser technique, optical fiber technique and tissue optics laid the groundwork for LIAF technology, which speed up its development rapidly in these years. The progress of most cardiac cancer patients had come to its medium or late term when they accepted examinations because of the early clinical situation nonspecificity of the illness. So the early diagnosis of cardiac cancer was a difficult problem all along. Fortunately, the diagnosis accuracy increased obviously as the invention and application of fiberendoscope. But the discovery rate in early stage was still lower than gastric cancer of other parts. The reasons come to the complication and variation of local anatomy and physiological function, the hiding growth mod of early lesion and the observation angle limitation of stomach-esophagus boundary. Therefore it's a method with developmentpotential and application prospect to associate LIF technology with existent endoscopy technique to apply to the diagnosis of cardiac cancer. The aim of this study was to find out the cardiac cancer microscopic autofluorescence character and the autofluorescence distribution and difference in every layer of normal gastric corpus tissue, investigate the cellular autofluorescence difference between carcinoma and normal gastric tissue, and have a preliminary discussion of the tissue microscopic fluorescence character and space distribution of fluorophore.Materials and MethodsA double-channel laser scanning confocal microscopy with Argon ion laser and Helium-Neon laser were used to detect the autofluorescence and analyse the images from 16 surgical specimens of cardiac cancer and corresponding normal gastric corpus tissues. And the detected fluorescence intensities were compared between carcinoma and normal tissues. Then the cardiac cancer and corresponding normal gastric corpus tissues were made into cell suspension. FACsort flow cytometer was used to detect the mean cellular autofluorescence intensity and the difference between the two kinds of tissue was analysed.Results1. Confocal microscopy studyThe most layers of nomal stomach wall including mucosa, submucosa and muscular layer can be distinguished distinctly by confocal microscopy. The fluorescence of mucosa was mainly emitted from the lamina proparia. In the laminal propria the signal was mostly located in the glands and was in the shape of spots or rarefaction bands. At the same time, fluorescence was difficult to observerd in the stroma. In the submucosa, the fluorescence signal was in continuous bandlike or netlike with high intensity. And in these three layers, autofluorescence intensity was strongest in the submucosa, in the muscularis layer was weaker and weakest in the mucosa lamina propria. There was significance in the differences by statistical analysis. F value of green fluorescence intensity was 47.158 by ANOVA method, and the red was 60.955. P value was lower than 0.001. And compared in every two layers the P values were all lower than 0.05 by Student-Newman-Keuls test. In the microscopic autofluorescence image of cardiac cancer, tissue constitution of every layer changed remarkably. The highly hyperplasia and infiltration of...