The Study Of Biology-comparability Of Chitosan/Protein/Calf Thymus DNA

The self-assembled modification of biomolecules on biomaterial, is an important task lately which can improve the biomaterial's consistent property. The consistent property among chitosan, protein and DNA was studied by atomic force microscope(AFM), FTIR etc, and some basal results were found.Based on representative chitosan among polysaccharides, hemoglobin(Hb) and bovine serum albumm(BSA), the composite and adsorptive property between chitosan and Hb/BSA were studied using better compatibility of chitosan. Chitosan and Hb self-assembled into the composite films with various layers at various pH, whose structures were studied by AFM and FTIR. The results showed that there is better biocompatibility between chitosan and Hb, and that this preliminary research will provide theoretic base for studies on Hb as a blood substitute in clinical application. On the study of chitosan and BSA, the molecular imprinting polymer with differential discriminating function was made, which could be used on separation and purification of biomolecules.In addition, AFM was used to investigate ultrastructure of calf thymus DNA by changing experimental conditions. Furthermore, calf thymus DNA damage induced by potassium dichromate in combination with Glutathione(GSH) was studied by AFM and UV. The resuks supported the medical conclusion in morphology and spectrography that GSH can induce potassium dichromate to generate various tumor-inducing intermediate products. Last, the interaction between protein and DNA was summarized, which can orient the relative study, for the future.