Effects Of Glycine On Lipid Metabolism In Mice And Its Inhibition For Hepatic Steatosis

OBJECTIVES: To observe the effects of glycine on lipid metabolism in mice and its inhibition for hepatic steatosis.METHODS: In the first part, mice were fed with high-fat diet for 12 days to establish hyperlipidemia , the effects of glycine on body fat content and serum lipid were observed, and the level of serum leptin were measured. In the second part, the same method as the first part to establish hyperlipidemia, the effects of 20% glycine on development of hepatic steatosis were observed, percent liver weight, activity of superoxide dismutase (SOD), liver malondialdehyde (MDA) content and liver protein content were measured, the liver samples were collected to make liver section.RESULTS: (1) Body fat content: body fat content in 20%GLY group was obviously lower than that in control group, and body fat content in HF+20%GLY group was obviously lower than that in HF group, and body fat content in HF+10%GLY group in female mice was obviously lower than that in HF group. (2) Serum lipid: the levels of TC and LDL in high-fat diet groups which were treated with GLY were obviously lower than those in HF group. (3) Serum leptin: the level of serum leptin in HF group were obviously higher than that in control group, the level of serum leptin in 5%GLY group and 10%GLY group in male mice were obviously higher than that in control group, the level of leptin serum in HF+5%GLY group in male and female mice were obviously higher than those in HF group. (4) SOD, MDA , liver protein: activity of SOD in HF+20%GLY group was obviously higher than that in HF group, MDA content was obviously lower than that in HF group, liver protein content was obviously higher than that in HF group. (5) Hepatic steatosis: hepatic steatosis in HF+20%GLY group was slighter than that in HF group.CONCLUSIONS: (1) Glycine decreased body fat content, correlating with the changes of leptin. (2) Glycine had no obvious effects on serum lipid in mice with normal diet, but it reduced the levels of TC and LDL in groups with high-fat diet. (3) Glycine prevented hepatic steatosis by reducing percent liver weight and MDA content, enhancing the activity of SOD and increasing liver protein content.