In Vitro Study Of Effect Of Arsenic Trioxide In Multiple Myeloma And Its Related Mechanism

OBJECTS To investigate the effect of arsenic trioxide(As₂O₃) on multiplemyeloma U266 cell line and the related mechanism in vitro.METHODS The viabilityand apoptosis of U266 cells were observed by MTT assay,flow cytometry (FCM),DNA electrophoresis and terminal-deoxynucleotidyl transferase mediatednick end labeling(TUNEL) respectively.The expressions of hTERT proteinand mRNA were assessed by western blot and RT-PCR analysis respectively.And wedetected the variation of procaspase-3 and bcl-2 protein expression through westernblot.The effect of As₂O₃ on the VEGF expression of U266 cells were studied byELISA and RT-PCR analysis.RESULTS The results showed that As₂O₃ inhibited thegrowth and viability of U266 cells and induced their apoptosis. As₂O₃ could inhibitthe expression of hTERT ,cause the collapse of the mitochondrial transmembranepotentials of U266 cells and activate the caspase-3,but had not effect on theexpression of VEGF and bcl-2.CONCLUSION The collapse of the collapse of themitochondrial transmembrane potentials of U266 cells,activation of caspase-3 anddown-regulation of telomerase activity by the downtrend of hTERT expression mayplay important roles in the apoptosis of U266 cells induced by arsenic trioxide.