| Objective: To investigate the effects of inhaled low dose nitric oxide (NO) on lungischemia-reperfusion injury during flush and delayed 10 minutes after reperfusion.Methods: Sixty health adult male Sprague-Dawley rats were randomly allocated toan NO and a control group. Before the donor lung was harvested, the right hilus wasclipped for 5 min (clipping test), then blood sample was collected from carotid arteryfor baseline arterial blood gas analysis. Graft was stored inflated at 4℃for about 6 hin LPD solution. Lung transplantation was performed in a modified "cuff-like"vessel anastomosis technique. Dynamic compliance (Cdyn) and resistance of airway(Raw) were monitored before operation (baseline) and after 2-h reperfusion. Thegraft's gas exchange and oxygenation were assessed by "clipping test"after 2-hreperfusion. The lung graft was harvested for measuring wet/dry weight ratio (W/D),myeloperoxidase (MPO) activity, inducible nitric oxide synthase (iNOS) activity andthe content of malondialdehyde (MDA).Results: After 2-h reperfusion, compared to the control group, PaO2, OI, Qs/Qt, anda/A were improved significantly in the NO group (277±91 vs. 157±47 mmHg,P<0.01; 2.67±0.89 vs. 4.72±1.48, P<0.01; 21.1±4.57 vs. 27.1±2.37, P<0.01;0.43±0.15 vs. 0.26±0.10 , P<0.05). The activities of MPO and iNOS weresignificantly reduced in NO group (1.80±0.46 vs 3.08±0.65 u/g.tissue,P<0.01;10.6±10.2 vs 97.8±82.2 pmol·mg protein-1·min-1,P<0.05). The content of MDAin the lung tissue of NO group was significantly higher than that of the control group(34.8±7.9 vs. 20.0±11.2 nmol/mgprot,P<0.05). Inflammatory cell infiltration wasalso significantly reduced (P<0.05). But there were not significant differencesbetween two groups in Cdyn, Raw and W/D ratio.Conclusion: We speculate that inhaled NO might prevent neutrophil sequestrationand the activity of iNOS in isograft, thereby ameliorated ischemia-reperfusion injuryand improved the oxygenation of the graft. |
PDF Full Text Request