Establishment Of Murine Model Of Bronchopneumonia Due To NTHi And NF-κB Expression In Lung

Objective To e stablish a m urine model o f bronchopneumonia due to NTHi and evaluate the role of NF-κB in the pathogenesis of NTHi bronchopneumonia. Method The murine model of NTHi bronchopneumonia used NTHi (ATCC 49247) encased in agar-beads as a bronchial inoculum. Control animals received NTHi-free agar beads. Animals were killed at intervals. Evaluate inflammatory score of the lung by histologic analysis. Total and differential cell count in BALF were determined. Detect the expression of nuclear factor-κB by immunohistochemistry method.Results Established a murine model of prolonged pulmonary infection due to NTHi that mimic the pathophysiology of human bronchopneumonia. Recovered NTHi from all the lungs till 5 days after infection. The inflammation was severest at day 5 and persisted for at least 2 weeks. An obvious induction of NF-κB nuclear translocation was observed in NTHi-agar and Agar group at day 1. The level of NF-κB in nucleus of bronchiole epithelium cell in NTHi-agar group at day 1 was higher than that in agar group accompanied by decreased expression in cell plasm. Conclusion Murine model of bronchopneumonia due to NTHi can be established by bronchial inoculation of organism encased in agar beads. NF-κB play a great role in the pathogenesis of lung inflammation resulted from NTHi.