Construction Of AFP Promoter Drived Lentiviral Vector And Human Fetal Liver Stem Cells Study

Stem cell has been studied for many years and has becaming one of the focuses in the world. And due to the increasing of liver disease and the lack of liver contribution, more and more scientist study liver stem cells. These studes will contribute to better understanding the characterization and treatment of engendering liver disease. And fetal liver is an excellent resource of liver stem cell. We study the separation, culture, inducing differentiation and tansplantation of human fetal liver cells through the following ways:1 To isolate the AFP positive fetal liver cells by FASC, we construct a recombinant lentiviral vector plv by replacing its CMV promoter to AFP promoter which can specially express EGFP in AFP producing cells. This selection system may give more support to study the characteristic of fetal liver stem cells and to the isolation and application of liver stem cells.2 It is hard to get enough cells for study or clinic trearment only by separating, and also it is unstable and expensive. The best way to solve this problem is to establish a stable cell line. In this study, we establish human fetal liver cell line by two ways: select the clonal fetal liver strains and coculture with feeder cells; immortalize fetal liver cells by using a retrovirus vector expressing the hTERT.3 To study the differentiation potential of human fetal liver cells, we tansplant it to NOD-SCID mouse and prove its ability of differentiatinng into several tissue cells.