Study On Influence Of SspA On Bacterial Biofilm To Thoracic And Cardiovascular Biomaterials In Vitro

Objective: To investigate the influence of SspA on the Staphylococcus aureus biofilm formation on Dacron Biomaterial surfaces under different SspA concentration.Methods: SspA was extracted and purified from the surface of Staphylococcus aureus, and then used to influence the adhesion of Staphylococcus aureus and the Staphylococcus aureus biofilm formation on the Dacron Biomaterial surfaces. Ninety Dacron Biomaterials were randomly assigned to 3 equal groups of 30 materials: in group A, Dacron biomaterials were randomly put into 30 vials contained 400ng/ml SspA and 105CFU/ml Staphylococcus aureus respectively; in group B, Dacron biomaterials were randomly put into thirty vials contained 80ng/ml SspA and 105CFU/ml staphylococcus aureus respectively; in group C, Dacron biomaterials were randomly put into 30 vials only contained 105CFU/ml Staphylococcus aureus respectively. The depth and number of Staphylococcus aureus biofilm on the surfaces of five Dacron Biomaterials were evaluated by confocal laser microscopy after incubating 6, 16,24, 30, 48 hours, and the shape of the Staphylococcus aureus biofilm formation was also evaluated by scanning electron microscopy at the same time.Results:In group C, the depths of the staphylococcus aureus biofilm on the Dacron biomaterials surfaces increased significantly with time dependence, and bacterial biofilm maturated after 48 hours incubation. In group A, bacterial biofilm on theDacron biomaterials surfaces were thinner than those in group B and group C at each time points (all P<0.05). In group B, the depths of bacterial biofilms on the Dacron biomaterials surfaces were significantly decreased at each time points as compared with those in group C(all P<0.05).The number of the Staphylococcus aureus biofilm formation on the Dacron biomaterials surfaces in group A decreased significantly at each time points as compared with those in group C and group B (all P<0.05). In group B, the number of the Staphylococcus aureus biofilm formation on the Dacron biomaterials surfaces was less than that in group C (all P<0.05).Conclusion: The study show that SspA can effectively inhibit the formation of Staphylococcus aureus biofilm on biomaterials surfaces with dose dependence. SspA therapy can provides a promising way on curing and controlling biomaterial centered infection.