The Establishment Of Quantitative Multiprobe PCR Assay For Diagnosis Of Bacterial Pathogens Of The Chronic Prostatitis

objective: We established a novel adaptation of the TaqMan PCR assay which potentially allowed for highly sensitive detection of any eubacterial species with simultaneous species identification. Our system relied on a Unprobe(unique multiprobe) designed in which a single set of highly conserved sequences encoded by the 16S rDNA gene served as the primer pair and was used in combination with both an internal highly conserved sequence, the universal probe, and an internal variable region, the species-specific probe(eg: ECprobe and SAprobe). medthods: we used Quantitative Multiprobe PCR to test the DNA of common bacterial pathogens of the Chronic prostatitis and used specific SAprobe and ECprobe to detect Staphylococcus aureus or Escherichia coli Results: The TaqMan system described reliably detected 10 common bacterial species with a detection limit of 50 fg(10~3). Further, highly sensitive and specific pathogen detection was demonstrated with a prototype species-specific probe designed to detect Staphylococcus aureus or Escherichia coli. This assay not only used detection and identification of Bacterial Pathogens of the Chronic prostatitis but also had broad potential in the clinical arena for rapid and specific diagnosis of infectious diseases.