Dynamic Study Of Induced Hepatocellular Carcinoma In Rats With Multi-Slices Spiral CT

(The First Affiliated Hospital of Kunming Medical College 650031) Objective :to produce an induced hepatocellular carcinoma model in rats.study the multi-slices spiral CT manifestation of HCC in the whole process of tumor progression and compare with histopathologic changes;try to find early signs of HCC on CT and provide an experimental evidence for early diagnosis and prognosis of HCC in clinic.Material:169 male Wistar rats were divided into 2 groups randomly, the administered group(n=112) and the control group(n=57);0.2% DEN.Method: the administered rats were orally administered DEN at concentration of 0.2%, 1ml per rat ,4~5 times a week in consecutive 16 weeks and rats of control were treated with saline in the same way.CT exams were performed at the end of 11~th 12~th 13~th. 17~th, 19~th, 21~st, 23~rd 24~th 25~th 26~th 27~th 28~th 29~th, 30~th week respectively. All rats were performed plain scan and 3-phase contrast enhancement scan. Rats were undergone pathologic exam immeadiately after CT exam.The specimens were treated by pathological method in conventional way.Results:cellular edema, fatty metamorphisis small foci of cellular necrosis ocurred at the end of 1~st week microscopically,and hepatocellular hyperplasia hepatocellular nodule, typical manifestation of cirrhosisoccurred in the order gradually;and the l~st HCC occurred at the end of 19th week.The ratio of successfully induced HCC is 76.6% from the end of 25thweek to the end of the experiment The lstHCC occurred on CT at the end of 19~th week and the lesions found on CT became more and more.There are S3 HCCs and 32 hepatocellular nodules found by pathological way,and in which,51 HCCs and 12 nodules were found by CT.HCCs on CT demonstrate various types of contrast enhancement,including no enhancement ring-like enhancement the whole enhancement irregular enhancemet The hepatocellular nodules demonstrate no enhancement Conclusion: The model of induced hepatocellular carcinoma in rats by means of orally administered small dose of DEN is suitable for imaging study for easily performing high ratio of inducing and simillar to human HCC; multi-slices spiral CT is applicable for examination of HCC in clinic. CT demonstration of induced HCC in rats are various,which is related to the components and the blood supply of tumor.