The Study Of Application Of Quantitative Multiprobe PCR Assay In The Pathogenic Diagnosis Of The Chronic Prostatitis

Objective:To investigate the potential association between prostatic bacterial infection and chronic prostatitis (CP),we report on the detection of bacteria in expressed prostatic secretions(EPS) or voided bladder 3 (VB3) using a quantitative multiprobe real-time PCR assay. Materials and Methods: Template DNAs were examined from expressed prostatic secretions(EPS) or voided bladder 3 (VB3) from patients with chronic prostatitis. One PCR primier sets that amplifies a portion of all known bacterial ribosomal DNAs(16SrDNAs) was udsed. Three fluorescent probes were used: one that detects all known bacteria (Uniprobe) , another detects Escherichia coli ( ECprobe ) and the last is specific for Staphylococcus aureus(SAprobe). Results:The 16SrDNAs real-time PCR assay detected bacteria DNAs in twenty eight(35%)of 80 samples from patients with chronic prostatitis, including forteen samples (17.5%)that were also positive by the E.coli real-time PCR assay and six samples (7.5%)that were also positive by the S.aurs real-time PCR assay . The count of white blood cell in EPS(VB3)of positive patients increases with ascension of concentration of bacteria and the two show linear correlation . But no correlation could be found between the quantity of bacterial concentration and the chronic prostatitis symptom index. ConcIusion:Quantification,speed and specificity make real-time PCR a promising approach for the quantitative detection and identification of EPS(VB₃) bacteria from chronic prostatitis patients.