The Effects Of Monoclonal Antibodies Directed Against The Human Ventricular Myosin Light Chain 1 (HVMLC1) On The ATPase Activity And Superprecipitation Of Cardiac Myosin.

Objective: To study the function of N-fragment and C-fragment of human ventricular myosin light chain 1. Methods: using expressed HVMLC1 as antigen, we have obtained three monoclonal antibodies, designated as C8, C9, B12. we examined whether monoclonal antibodies combined with the antigen and validated the case monoclonal antibodies combined with N-fragment and C-fragment of the antigen through westen-blot method, we also inspected the effects of monoclonal antibodies on ATPase activity and superprecipitation of cardiac myosin. Result: Immunoblot experiments demonstrated that all three monoclonal antibodies could combine with the antigen, C8 combined with the NN part of N-fragment of the antigen, both C9 and B12 combined with the C-fragment of the antigen. In the presence of C9 and B12, ATPase activity and superprecipitations of myosin was greatly inhibited, while there was almost no effect on the ATPase activity and superprecipitations for C8. conclusion: These findings not only confirm the C-fragment of HVMLC1 is the modulated domain of myosin, it plays an essential role in the interaction of actin and myosin, but also demonstrated that the native intact myosin molecules does not hinder the access of antibody molecules to the epitopes of C-fragment of LC1.