Study On The Biological Mechanism Of Arsenic And Fluoride In Human Lymphocytes In Vitro

Objective : To investigate the biological effect and function mechanism of sodium arsenite (NaAsO₂) and sodium fluoride (NaF) in human lymphocyte in vitro and provide the basis for further study. Methods: The action of NaAsO₂ and NaF on the cell growth, the morphologic alteration, DNA damage and interleukin-2 (IL-2) secreting in human lymphocyte in vitro was observed by MTT assays, transmitting electro microscope (TEM) detection, single cell gel electrophoresis (SCGE) and enzyme linked immunosorbent assay. Results: It is shown from our study that NaAsO₂ and NaF has a remarkable inhibitory effect on the proliferation of human lymphocytes with the concentration of 1μmol/L and 50μ mol/L respectively (P<0.05). The results of observations by TEM showed that the morphologic alteration of lymphocytes was occurred after NaAsO₂ and NaF treatment. The concentration of 1μ mol/LNaAsO2 and 50 u mol/L NaF showed significant damage on DNA and inhibition on IL-2 secreting^ < 0.05). The interaction of NaAsCb and NaF was not found in this test(P>0.05). Conclusion: Both arsenic and fluoride have a poisoning effect on human lymphocytes with lower dose, but the interaction in the combination group was not found, it was simply additive.