The Significance Of Detection Of Chemokines In Serum Of Infants With Respiratory Syncytial Virus Infection

Objective: To explore the roles of macrophage inflammatory protein-1alpha (MIP-1α) and regulated upon activation normal T cell expressed and secreted(RANTES) in the pathogenesis of respiratory syncytial virus(RSV) infection, and to explore the correlation of the concentrations of MIP-1α and RANTES in serum and airway hyperresponsiveness,Methods: Serum samples were obtained from 45 infants with RSV infection, including 10 bronchial asthma , 15 bronchiolitics or pneunonia , 20 upper respiratory tract infection ; 20 healthy infants with non- RSV infection as the normal group. ELISA method was used to determine the concentrations of MIP-1α and RANTES in serum.ResultS: MIP-1α and RANTES levels in infants with RSV infection were much higher than those of non- RSV infected healthy subjects (P<0.05 or P<0.01). MIP-1α and RANTES levels of bronchial asthma and those of severe RSV infection subjects were much higher than upper respiratory tract infection subjects. But these was no significant different between bronchial asthma and those of severe RSV infection group. It was also found that serum concentrations of MIP-1α and RANTES were positively correlated to the number of eosinophilis.Conclusions: The concentrations of chemokines of MIP-1α and RANTES in serum will increase after respiratory syncytial virus infection, leading to eosinophilis recruiting, lung inflammatory and airway hyperresponsiveness, which may be the pathological base for RSV infection to bronchitis asthma .