Effect Of Rolipram On The Airway Intraepithelial Dendritic Cells In Murine Models

Objective: To investigate the effect of selective PDE4 inhibitor(rolipram) on the number of airway intraepithelial dendritic cells in murine models; To compare the effect of rolipram on the number of airway intraepithelial dendritic cells in murine models with aminophylline; To investigate the effects of different concentration of rolipram on the number of airway intraepithelial dendritic cells in murine models.Methods: 48 of BALB/c mice were randomly divided into six groups: control group, asthma group, aminophylline-treatment group and rolipram-treatment group (low, middle, high concentrations). The animal models of asthma was established by OVA sensitizing-challenge. At 24h after the last challenge, the pulmony and tracheal samples were performed. Immunohistochemitry was used to detect DC by horseradish peroxidase staining using the NLDC-145 monoclonal antibody. The computer image analysis were applied to analyse DC quantitatively.Results: The number of NLDC-145~+DC in the bronchial mucosa of asthmatic mouse(210±35.6/mm~2)is more than that in healthy controls (p<0.01); After seven days treatment with rolipram, the number of NLDC-145~+DC in low and middle concentration groups is(90±24.4, 88±24.3个/mm~2 ) less than that in asthmatic group (p<0.01), and the number of NLDC-145~+DC of rolipram-treatment group (low, middle ) is less than that in aminophylline-treatment group(130±22.8/mm~2) (p<0.01); While in high concentration group (200±35.4/mm~2) , there were no obvious change (p>0.05).Conclusion: It is suggested that dendritic cells are involved in the inflammation of airway of asthmatic murine and that rolipram may down-regulate the number of dendritic cells much more than aminophylline; Down-regulate the number of dendritic cells may be one therapy-mechanism of rolipram and aminophylline.