Experimental Study On Mononuclear Cells From Human Umbilical Cord Blood Differentiating To Hepatocyte-like Cells In Vitro

To investigate the feasibility of induction mononuclear cells (MNCs) from human umbilical cord blood to hepatocyte-like cells with cytokines in vitro. In vitro differentiation mononuclear cells (MNCs) isolated and purified from human umbilical cord blood to hepatocyte-like cells with SCF OSM HGF LIF, FGF-1 FGF-2. Half quantitative RT-PCR and immunocytochemistry was performed to observed the expression of hepatocyte markers . The study indicated that mononuclear cells (MNCs) from human umbilical cord blood can be differentiated to hepatocyte-like cells in vitro with these cytokines; mononuclear cells (MNCs) from human umbilical cord blood can't be differentiated to hepatocyte-like cells in vitro without cytokines.