The Effect Of Sleep Deprivation On Cognitive And Cerebral Mitochondrial Respiratory Function In Rats

Objective To investigate the effects of various degrees of sleep deprivation on cognitive and cerebral mitochondrial respiratory function in rats. Methods All the 50 SD rats were subjected to a 10-day sleep deprivation protocol and were divided into 5 groups randomly: allowing 12h of sleep every day (control group), allowing 9h of sleep every day (group 1) , allowing 6h of sleep every day (group2) , allowing 3h of sleep every day (group3) , allowing Oh of sleep every day (group4) . The modified multiple platform method (MMPM) was used to establish sleep deprivation model. The cognitive function was tested by Y-type maze. Mitochondrial respiratory function was measured by Clark-type oxygen electrode. Results The error reaction numbers (ENs) were significantly increased in sleep deprived rats. There were statistically significant differences (p<0.05 ) in EN between the control group and the completely deprived (Oh group) or severely deprived (3h group) rats on each of the study days. The oxygen consumption rate in state 3 respiration was significantly decreased (p<0.05) in sleep deprived rats compared with the control group. The oxygen consumption rate in state 4 respiration was significantly decreased (p<0.05 ) in the completely deprived (Oh group) rats compared with the control group. Conclusion Sleep deprivation, no matter mild or severe, both could have adverse effects on cognitive function and mitochondrial respiratory function.