Purification Of The Type â…¡ Collagen From Sheep Cartilage And Its Effection On Adjuvant Arthritis In Mice

This paper is divided into two parts : Purification of the Type â…¡ Collagen(C â…¡) from Sheep Cartilage and its effect on Adjuvant Arthritis(AA). At first, the Câ…¡ was purificated from sheep cartilage by using enzymatic hydrolys method;Secondly, Its effect on rheumatoid arthrit was studied by oral type II collagen in KM mice.After removel of the periosteum from sheep cartilage, the proteoglycans were extracted by guanidinium hydrochloride and dissolved by pepsin in acid solvent.Finally the floccule obtained by salting-out from the supernatant was Câ…¡ .The result of SDS-PAGE was suggested that the bands of Câ…¡ samples and standard Câ…¡ were at the same location,and the purity was 93.12%.The classical CFA inducement method of RA animal model was adopted .The results of pre-tests showed that the best dosage was 0.6 ml/ d every mice. Sixty KM mice were selected with similar weight and age,and randomly divided into four groups: control group (group A), prevention group (group B), therapy group (group C), model group (group D). Every group was determined the weight gain once a week, the thickness of the sole every three days after inflammation, the blood serum indices and the weight of organs on the 24^th after inflammation.The results were followed: (1) The trend of weight was that group A had no significant difference;group B increased slowly after three days of induced inflammation;the obvious trend for group C was not found at the beginning ,but the trend was increasing significantly after ten days of induced inflammation;the trend of group D decreased at first and then increased slowly. (2) The severity of inflammation in AA mice of group B significantly decreased after twelve days of induced inflammation;the severity of inflammation in AA mice of group C decreased after fourteen days of induced inflammation;the severity of sole in the primary inflammatory side of group D were aggravated all along , the severity of sole in secondary inflammatory side were aggravated at first and then alleviated after eighteen days of induced inflammation. (3) Compaired with group A, group D enhanced significantly the value of IL-1 (p<0.01);the value of group B was significantly lower than that of group A (p < 0.05);group A had no significant difference copared with group C (p > 0.05) .It indicated that group B and C could alleviateinflammation, and the former is better than later. (4) The value of NO in serum was significantly higher than group A (p<0.01) ,but group B and C significantly lower than group A seperately (p<0.01) . (5) Compaired with group A , group D decreased significantly the value of SOD(p<0.01 )and enhanced significantly the value of MDA (p<0.05);the value of group B and C significantly higher than group A seperately (p <0.01). (6) The weight of liver changed significantly as the weight of the immune organs was compaired : group A was significantly lower than group B (p<0.01);group A had no significant difference compared with group C (p>0.05);group B was significantly higher than other groups seperately (p<0.01) .It indicated group B had the best effect.