Studies On The Bioactive Constituents From Stichopus Variegatus Semper

Guided by Pyricularia oryzae bioassay model, the anticancer and antifungal constituents of Stichopus variegatus Semper have been investigated by various chromatography methods including LPLC, MPLC, HPLC on silica gel,ODS RP C-18 and Zorbax SB C-18 respectively.Ten compounds were isolated and structures of seven compounds were elucidated by chemical and spectral analysis (IR, EI-MS, ESI-MS,HRESI-MS, ~1H NMR, ~13C NMR, ~1H-~1H COSY, DQF-COSY, TOCSY, HMQC, HMBC, NOESY).These compounds showed moderate cytotoxity in vitro against human tumor cell lines such as A-549,P-388.Our studies focused on bioactive constituents of Stichopus variegatus Semper have established a foundation for further research and development of the kind sea cucumber with abundant resources in South China Sea, and provided important leading compounds for the development of new anti-cancer and anti-fungus drugs.