The Study Of Amino Acid Imbalance Solutions On Hepatocarcinoma Inhibition And Organism Nutrition Status Influence

Objectives: To observe the change of free amino acid metabolism in Hepatoma22-bearing mice and support theoretic base for amino acid imbalance therapy . Methods: Level of 10 free amino acid in plasma and 10 in tumor tissue of Hepatoma22-bearing group were measured using HITACHI L-8800 analysis instrument comparing with normal group to study the change of free amino acid metabolism and analyze the correlations. Results: The plasma free arginine. valine, serine and glutamic acid were significantly decreased in hepatoma-bearing group. The negative correlation was found between the tumor tissue free methionine, arginine, valine, leucine and the corresponding plasma free amino acid. The positive correlation was found between the tumor tissue free isoleucine, valine, methionine, tyrosine, leucine, glutamic acid, serine and tumor volume. Conclusions: Somespecific amino acids have the close relation with hepatocarcinoma growth. These will provide the laboratorial and theoretic base for the constitution of amino acid imbalance solution.Objectives: To study the influence of several amino acid imbalance solutions on hepatocarcinoma inhibition and nutrition status in tumor-bearing mice. Methods: Total gastric-intestinal nutrition solutions had been used for 12 days in Hepatoma22-bearing mice. According to formulas of amino acid composition in nutrition solutions respectively, the hepatoma22-bearing mice were divided into seven different groups: group A(amino acid balance solution^ group B(arginine-enriched amino acid imbalance solution^ group C(valine-depleted amino acid imbalance solution) ^ group D(leusine-enriched amino acid imbalance solution) ^ group E(tyrosine-depleted amino acid imbalance solution) > group F(phenylalanine-depleted amino acid imbalance solution^ group G(serine-depleted amino acid imbalance solution). Indices of hepatocarcinoma inhibition include tumor volume > tumor weight> tumor weight/carcass body weighs necrotic rate of tumor celk PCNAandDNA ploidy. Indices of nutrition status include weight lost> transferrin(Trf) > prealbumin(PA) and T-cell subgroup. Results: All tumor inhibition indices of group B, C> D, E> F> G were significantly less than those of group A except necrotic rate of tumor cell and DNA ploidy. The levels of weight lost were significantly more in group C^D^E^F^G than those in group A, B. The levels Trf and PA in these groups were not found significantly differences. The level of T-cell subgroup in group B was significantly highly than that in group A. Conclusions: Amino acid balance solution can stimulate tumor growth, whether all amino acid imbalance solutions have similar inhibition effects;Amino acid balance solution and arginine-enriched amino acid imbalance solution can reserve nutrition status of tumor-bearing mice relatively, whether other amino acid imbalance solutions have similar disadvantageous influence.