Cardioprotective Effects Of Ginaton On Vivo Ischemia-reperfusion Rabbits

Cardioprotective Effects of Ginaton on vivo Ischemia - reperfusion RabbitsIntroductionIntrinsic cardioprotective mechanisms are welcome up to date, HSP70 is one of these mechanisms, and it is one of cardioprotective mechanisms in myocardial ischemic preconditioning and pharmacological preconditioning yet. Myocardial is-chemic preconditioning is unprobable in clinic,but pharmacoloical preconditioning is safe and uninvaded, therefore this study is based only on the above theory to observe Ginaton injection's cardioprotective mechanisms.Materials and Methods30 rabbits with ischemia for 30 minutes received saline or Ginaton reperfusion intravenously for 1h,3h and 5h,respectively, there were 5 rabbits at every time point. The other 5 rabbits without ischemia/reperfusion (I/R) were used as normal control group. Western blot was used to measure HSP70, the content of HSP70 was analysed semiquantitatively. Serum Total Superoxide Dismutase ( T -SOD) , Malondiadehyde ( MDA) and Creatine Kinase ( CK) were measured at end.ResultsThere was less expression of HSP70 in normal control group, the ratio of ischemic to non - ischemic area was 28. 12 ± 0. 73 to 23. 42 ± 0. 54, P < 0.01. There was obvious expression in saline reperfusion for 3h and in Ginaton reperfusion for 1h,the ratio of ischemic to non - ischemic area was 69. 92 ±1. 19 to 49.41 ±0.7 (P<0.01) and 373.92 ±4.09 to 275.98 ±4.91 ( P <0. 05) , respectively.The expression increased gradually with reperfusion time by folds. Serum T - SOD level obviously descreased and MDA level obviously increased in saline group, Serum T - SOD level slightly decreased and MDA level slightly increased only in Ginaton reperfusion, there were statistic values in two groups at the same time pionts( P<0.05 orP<0.01). Serum CK level slightly changed in Ginaton reperfusion, but obviously increased in saline reperfusion, the statistic values were obvious in two groups at the same points(P <0.05 orP<0.01).DiscussionHeat shock protein 70 is a kind of adaptive protein, it only expresses when living cells face stress. Repeated and transient ischemic can lead to myocardial resistance to subsequent prolonged ischemia, namely ischemic preconditioning. Based on ischemic preconditioning, pharmacological strategies for the limitation of ischemic myocardial injures are adopted in clinic, namely pharmacological preconditioning. The present study has employed in vivo myocardial ischemia model to assess ischemia - reperfusion insult by T - SOD, MDA and CK, and employed Ginaton injections pharmacological preconditioning to induce HSP70s expression for observing cardioprotective mechanism on the level of cell and molecule. The study showed HSP70 can only obviously express in 3 hours after ischemic — repfusion, not only in ischemic area but also in nonischemic area, and gradually increased by folds with repfusion time. Ginaton injection can induce HSP70 to express early, in 1 hour after ischemic - repfusion, foremore expressed excessively, which lead to obvious acute phase's anti - oxidation and cardioprotection by comparing with saline repfusion. Foremore, Ginaton en-chanced the level of T - SOD and decreased the level of MDA, and increased the ability of anti - oxidation and decreased myocardial injure. This suggested Ginaton injections cardioprotion and anti -oxidation may related to HSP70.ConclusionHSP70 expresses obviously at 3h after I/R;Ginaton injection can induce expression of HSP70 early and excessively, leading to protect cardiac tissue;Gi-naton^ anti - oxidation may relate to HSF70.