Comparison Of The Field Strains And The Delta-methrin Susceptible Strains Of Musca Domestica Based On Proteome

Recently, proteomic analysis technology has been used extensively in biological innovative researches and has become one of the most essential technologies in functional genomics. The proteomics is the study of various protein properties (expression level, post-translation modification, etc) at the whole cell level ever whole body to obtain a global physiological and biochemical processes of cells and regulatory networks at the protein level. Proteomic technology has also been applied in studying protein function. By using proteomic technology, it has become possible to analyze simultaneously hundreds or even thousands of protein. The housefly (Musca domestica) is an important vector in the dissemination of various infectious diseases such as cholera, shigellosis and salmonellosis. In order to further elucidate the biochemical mechanism responsible for insecticide resistance in the housefly, this paper aim to display the differential proteins of adult flies of the field strain and the delta-methrin susceptible strain based on two-dimensional liquid chromatography and two-dimensional gel electrophoresis technologies. This results are as follows:1. By comparing of protein profiles in the field strain and the delta-methrin susceptible strain using the PF-2D, the results are: (1) 626 protein peaks were detected in the field strain, there were 251 protein peaks in pI>8.4, 228 protein peaks in8.4≤pI≤4.2, 132 protein peaks in pI<4.2;(2) 734 peaks were detected in the delta-methrin susceptible strain, there were 237 protein peaks in pI>8.4, 303 protein peaks in 8.4≤pI≤4.2, 168 protein peaks in pI<4.2. Of all protein peaks compared field strain to the delta-methrin susceptible strain, 99 protein peaks changes than two times . Of which changed peaks compared to the delta-methrin strain, 43 proteins up-regulation and 56 down-regulation in the field strain. The protein peaks between 8.4 and 4.2, 14 proteins up-regulation and 48 down-regulation in the field strain.2. The differential display of the housefly proteins are gained based on the two-dimensional gel electrophoresis technology. 339 protein spots were detected in the field strain by silver staining, in which 94.44% spots in pI 8≤pI≤4. 450 protein spotswere detected in the delta-methrin strain, in which 94.98% in p/ 8^pJ^4. in, There are 16 proteins up-regulation and 52 down-regulation in p/between 8.00 and 4.0 for the field strain.3. Summarize the results of the entire study, 1319 protein peaks are separated with the PF-2D, 789 protein spots with 2-DE. Among of them, 611 protein peaks and 339 protein spots are gained In the field strain, 708 peaks and 450 protein spots are gained in, the susceptible strain. The components with PF-2D separation are more 530 proteins than the 2-DE separation. In p/ 4-8 region, the protein spots of the susceptible strain are more 69 proteins than the field strain. In pi 4.2-8.4 region, the protein peaks of the susceptible strain are more 75 proteins than the field strain. These results suggested that some protein repressed in the field strain, these protein should get further identification.