Investigation Of The Relationship Between The Perienchyma Of Gliomas Infiltrating Dendritic Cells And The Bionomics Of The Tumor

Objective To investigate the dependability between the perienchyma of glioma infiltrating dendritic cells and the patho-classify of the gliomas and the proliferating of the tumor cells. To research the availability of dendritic cells-based immunotherapy for gliomas and prognosis evaluate. Method The 36 examples of gliomas were discriminated by WHO pathoclassify and signed on PCNA by immunohistochemical staining. The dendritic cells were signed by Fitc\Tritc immunofluorescence double staining. The amount of positive cells were counted using microscope and the cell index were calculated. Result (1) There were fluorescein stain on DCs cellular membrance. The number of DCs were less in perienchyma of gliomas than in normal cerebral tissue(P<0. 01). (2) The number of DCs were different between low potential malignancy and high potential malignancy .The number of DCs in low potential malignancy(WHO I \II) were much more than the index of DCs in high potential malignancy (WHO III \ IV) (P<0.01). (3)The number of DCs negatively correlated with WHO patho-claccify(P<0. 01). (4)PCNA index in perienchyma of gliomas were much more than that in normal cerebral tissue (P<0. 01). (5)The index of PCNA in high potential malignancy(WHO I \II) were much more than the index of DCs in low potential malignancy(WH0III\IV) (P<0. 01). (6)PCNA index positively correlated with WHO patho-claccify(P<0. 01). (7)The number of DCs negatively correlated with The index of PCNA (P<0. 01). Conclusion The number of DCs can judge the cell different degree of glioma and the proliferating of the tumor cells. It can evaluate the prognosis of tumor therapy. The immunotherapy of glioma can depend on DCs.