Study On Apoptosis Induced By Epirubicin And Chlorpromazine In Human Leukemia Cell Line Jurkat

Malignant tumors are not only related to abnormal proliferation and differentiation,but also to unconventional cellrlar apopotosis.Therefore,it is a good strategy to treat oncoma by means of interveniently Modulating oncocytic apoptosis because of its unbalance.Now,one of Functionary mechanism for many methods to treat malignant tumor used in clinic,such as radiotherapy,chemotherapy,hormonetherapy,heat-therapy,and so on,is toinduce apoptosis.Nowadays,chemotherapy has been one of three main methods to treat malignant oncoma in the way of adjuvant therapy.It can be coordinate with surgery and radiotherapy and be in transition from the level of palliative chemotherapy to radical chemotherapy gradually.Many kinds of anticarcinogen drugs may cause oncocytic apoptosis,so we can say that apoptosis induction may be the major mechanism in chemotherapy.This study is to observe apoptosis phenomena of Jurkat cell line,which belongs to one type of T-cell leukemia.Epirubicin and chloropromazine were applied to Jurkat cell.I plan to choose three steps to observe apoptosis :MTT colorimetric method to assess cytoinhibition ratio;electron microscope to observe morphological change of cell apoptosis ;flow cytometry analysis techniques(FCM) to measure cell cycle and apoptosis ratio.