Effects Of Artifical Progesterone On The Morphology Of Ovaries And Uteri And Development Of Hamster Embryo

Objective:To explore morphological changes in ovaries and uteri, expressionalchanges of Lectin receptors in uterine endometria and the mechanism ofabnormity in development of hamster embryo after using artificalprogesterone, we use pregnant hamster injected with progesterone asmodel systems to do five experiments.Methods:1. observe the structure of ovaries and uteri under conventional lightmicroscopy and transmission electron microscopy.2. examine the normal distribution and dynamic changes of Lectinreceptors in endometria with affinity cytochemistry and imageanalysis.3. measure semiquantificationally the content of ConA, PNA, UEAreceptors with immunoblotting.4. analysis the chroma of FSH, LH, E2, P in serum withradioimmunoassay.observe the resuming of reproductive cycle after stopping usingprogesterone.Results:1. there are great morphological changes in ovries and uteri undermicroscopy. The bulginess in uteri tends to diminish. The epithelialcells become shorter and arranged mussily. The cilia of the cellsdisappeared. There are bleeding in lamina propria of the endometria.2. the level of ConA, PNA, UEA receptors changes greatly in endometria6 days after stopping using progesterone. Dense brown particles canbe seen along the free surface of endometria, which prove to bestrongly positive.3. the content of ConA, PNA, UEA receptors in experimental group ismuch higher than that in control group.4. the chroma of FSH, LH, E2, P in serum is quite different between theexperimental group and the control group.1~6 days serum chroma after stoping drug (x±s n=20)group (d) FSH (IU/L) LH(IU/L) E2(ρ/μg·/.L-1) P (ρ/μg·/.L-1)control 2.94±0.024 * 1.54±0.048 * 7.1±0.024 * 0.25±0.041*treated 1 1.76±0.011 122±0.029 6.95±0.003 0.22±0.0162 1.34±0.013 1.12±0.052 5.65±0.004 0.21±0.0163 1.16±0.015 1.10±0.039 5.25±0.002 0.12±0.0154 1.06±0.011 1.08±0.022 5.13±0.021 0.07±0.0125 0.96±0.024 0.82±0.032 5.05±0.012 0.04±0.0216 0.76±0.013* 0.22±0.059* 4.95±0.007* 0.02±0.001**P < 0.055. the reproductive cycle is resumed 10 days after stopping usingprogesterone, which substantiate that the female mice recovered theability to procreate. This experiment also proves that inhibitory action ofartifical progesterone can be removed after stopping using progesterone.Conclusion:Artifical progesterone inhibit the secretion of ganadotropin releasinghormone by degenerative feedback to hypothalamus, which decrease thesecretory function of ovaries and make uteri abnormal. We can seemorphological abnormity in endometria under light microscopy andelectron microscopy. At the same time, on the free surface of endometria,the synthesization of Lectin receptors increase greatly. The normalcontent and distribution of Lectin receptors are indispensable forimplantation and embryo development. Artifical progesterone can inhibitthe survival of embryo because the abnormal expression of Lectinreceptors blocked the information transfer and misled the recognitionbetween embryonic cells and endometrial cells, resulting in the failure ofadhesion and orientation.Our studies also showed that the decrease of serum chroma ofprogesterone changed the microenviroment in uteri by affecting theexpression pattern of Lectin receptors. The reproductive cycle is resumed10 days after stopping using progesterone, which implies that inhibitoryaction of artifical progesterone is reversible.